Connexin 43/GJA1 antibody (C-Term, Intracellular)

Catalog No. ABIN7043198

Product Details anti-Connexin 43/GJA1 Antibody

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Binding Specificity: AA 331-345, C-Term, Intracellular

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Characteristics: Anti-Connexin-43 Antibody is directed against an epitope of human Connexin-43. Anti-Connexin-43 Antibody (ABIN7043198, ABIN7044079 and ABIN7044080)) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize Cx43 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Connexin-43 (GJA1 Products)

Background: Alternative names: Connexin-43, CX43, Gap junction alpha1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein

Gene ID: 2697

NCBI Accession: NM_000165

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization