GIPC1 antibody (Intracellular)

Catalog No. ABIN7043215

Product Details anti-GIPC1 Antibody

Target: GIPC1 (GIPC PDZ Domain Containing Family, Member 1 (GIPC1))

Binding Specificity: AA 10-24, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIPC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to GIPC1

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - 14,15 amino acid residues identical

Characteristics: Anti-GIPC1 Antibody (ABIN7043215, ABIN7045188 and ABIN7045189) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GIPC1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KAPPLVENEEAEPGR, corresponding to amino acid residues 10-24 of human GIPC1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235592)

Blocking Peptide: (ABIN7235592)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GIPC1

Target: GIPC1 (GIPC PDZ Domain Containing Family, Member 1 (GIPC1))

Alternative Name: GIPC1 (GIPC1 Products)

Background: PDZ domain-containing protein GIPC1, GIPC, GAIP C-terminus-interacting protein, RGS-GAIP-interacting protein, Synectin, NIP, TIP-2,GIPC1 belongs to a family of evolutionarily conserved adaptor proteins responsible for the trafficking of numerous transmembrane proteins via endocytosis. Members of GIPC (GAIP interacting protein, C-terminus) have three main domains responsible for protein interactions, a PDZ domain which is flanked by GH1 and GH2 domains. GH1 domain is responsible for the dimerization of the protein, while GH2 is important for the interaction of GIPC1 with MYO6, a retrograde motor protein involved in protein trafficking. The PDZ domain of GIPC1 is responsible for the protein to interact with numerous proteins, namely β1-adrenoceptor, D2 dopamine receptor, GLUT1, TrkA receptor just to name a few. The zebrafish ortholog also interacts with Neuropilin-21,2.GIPC1 expression is upregulated in breast, ovarian and pancreas cancers2.

Alternative names: GIPC1, PDZ domain-containing protein GIPC1, GIPC, GAIP C-terminus-interacting protein, RGS-GAIP-interacting protein, Synectin, NIP, TIP-2

Gene ID: 10755

NCBI Accession: NM_005716

UniProt: O14908

Pathways: Dicarboxylic Acid Transport