GRID2 antibody (Extracellular)

Catalog No. ABIN7043236

Product Details anti-GRID2 Antibody

Target: GRID2 (Glutamate Receptor, Ionotropic, delta 2 (GRID2))

Binding Specificity: AA 206-218, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRID2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Glutamate Receptor δ2 (GRID2)

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse - identical

Characteristics: Anti-GRID2 (extracellular) Antibody (ABIN7043236, ABIN7044376 and ABIN7044377) is a highly specific antibody directed against an epitope of the rat Glutamate receptor δ2. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize GluD2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KMITTLFDTMRIE, corresponding to amino acid residues 206-218 of rat GluD2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:600

Comment:

Negative Control: (ABIN7235649)

Blocking Peptide: (ABIN7235649)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRID2

Target: GRID2 (Glutamate Receptor, Ionotropic, delta 2 (GRID2))

Alternative Name: GRID2 (GRID2 Products)

Background: Ionotropic glutamate receptor δ2, GluR delta-2 subunit, GluD2,Excitatory neurotransmission in the vertebrate central nervous system is mainly mediated by ionotropic glutamate receptors (iGluRs). Molecular cloning identified 18 mammalian iGluR subunits, of which only 16 sort into the traditional pharmacological subfamilies of AMPA, kainate (KA), and N-methyl-D-aspartate (NMDA) receptors. The 2 remaining subunits were termed "orphan" receptors, "glutamate-like" receptors, "nonionotropic" receptors, or, most commonly, delta receptors1.Ionotropic glutamate receptors are integral membrane proteins composed of four large subunits that form a central ion channel pore. Sequence similarity among all known glutamate receptor subunits, including the δ receptors, suggests they share a similar architecture. Glutamate receptor subunits are modular structures that contain four discrete semiautonomous domains: the extracellular amino-terminal domain (ATD), the extracellular ligand-binding domain (LBD), the transmembrane domain (TMD), and an intracellular carboxyl-terminal domain (CTD)2. The delta family of ionotropic glutamate receptors (iGluRs) consists of the glutamate δ1 (GluD1) and glutamate δ2 (GluD2) receptors3. GluD1 is highly expressed in the inner hair cells of the organ of Corti4, diffusely expressed throughout the forebrain during development with high levels in the hippocampus during adulthood3. Deletion of GluD1 leads to a deficit in high frequency hearing in mice5. Genetic association studies have established the GRID1 gene, which codes for GluD1, is a strong candidate gene for schizophrenia, bipolar disorder, and major depressive disorder6. Copy number variation studies have also implicated GRID1 in autism spectrum disorder (ASD)7. In addition, GRID1 gene is localized to the 10q22-q23 genomic region which is a site for recurrent deletions associated with cognitive and behavioral abnormalities8.

Alternative names: GRID2, Ionotropic glutamate receptor delta2, GluR delta-2 subunit, GluD2

Gene ID: 79220

NCBI Accession: NM_001286838

UniProt: Q63226

Pathways: Synaptic Membrane