GRIK4 antibody (Extracellular)

Catalog No. ABIN7043240

Product Details anti-GRIK4 Antibody

Target: GRIK4 (Glutamate Receptor, Ionotropic, Kainate 4 (GRIK4))

Binding Specificity: AA 49-61, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Kainate Receptor GluK4

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse - identical

Characteristics: Anti-GRIK4 (KA1) (extracellular) Antibody is directed against an extracellular epitope of the rat kainate receptor GluK4. Anti-GRIK4 (KA1) (extracellular) Antibody (ABIN7043240, ABIN7044380 and ABIN7044381) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize GluK4 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KNRINRAPERLGK, corresponding to amino acid residues 49-61 of rat kainate receptor GluK4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235665)

Blocking Peptide: (ABIN7235665)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRIK4

Target: GRIK4 (Glutamate Receptor, Ionotropic, Kainate 4 (GRIK4))

Alternative Name: GRIK4 (GRIK4 Products)

Background: Glutamate receptor ionotropic kainate 4, GluK4, Glutamate receptor KA-1,Glutamate is the principal excitatory neurotransmitter in the central nervous system (CNS). Glutamate is involved in cognitive functions like learning and memory. Imbalances in glutamatergic transmission have profound physiological and behavioral consequences1.Ionotropic glutamate receptors are classified functionally and by molecular homology into three receptor classes: N-methyl-D-aspartate (NMDA), amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), and kainate (KA)2. KA receptors assemble as tetramers from five subunit types, GluK1, GluK2 GluK3, GluK4 and GluK5. The GluK1-GluK3 subunits have low glutamate affinity and are capable of forming functional homomeric channels. GluK4 and GluK5 are high-affinity kainate receptor subunits that bind glutamate but require coassembly with one or more GluK1-GluK3 subunits to form functional channels. The heteromultimeric assembly of kainate receptors like many other ion channels leads to the formation of receptors with unique pharmacological and functional properties3. Unlike the other KA receptor subunits, which are expressed throughout the CNS, GluK4 expression is limited to only a few regions of the brain, and expression is highest in the CA3 region of the hippocampus and dentate gyrus4.The GluK4 receptor subunit gene, GRIK4, is located near the tip of the long arm of chromosome 11. Genetic variants in the GRIK4 gene have been demonstrated to associate with several psychiatric disorders. GRIK4 has been identified as a susceptibility gene in schizophrenia and bipolar disorder5. GluK4 may also play a role in excitotoxic neurodegeneration6.

Alternative names: GRIK4 (KA1), Glutamate receptor ionotropic kainate 4, GluK4, Glutamate receptor KA-1

Gene ID: 24406

NCBI Accession: NM_014619

UniProt: Q01812

Pathways: Synaptic Membrane