Metabotropic Glutamate Receptor 4 antibody (Extracellular)

Catalog No. ABIN7043249

Product Details anti-Metabotropic Glutamate Receptor 4 Antibody

Target: Metabotropic Glutamate Receptor 4 (GRM4) (Glutamate Receptor, Metabotropic 4 (GRM4))

Binding Specificity: AA 33-44, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 4

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-mGluR4 (extracellular) Antibody is directed against an extracellular epitope of rat mGluR4. Anti-mGluR4 (extracellular) Antibody (ABIN7043249, ABIN7044344 and ABIN7044345)) can be used in western blot and live cell imaging applications. It has been designed to recognize mGluR4 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: KPKGHPHMNSIR(C), corresponding to amino acid residues 33-44 of rat mGluR4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GC014

Blocking Peptide: BLP-GC014

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Metabotropic Glutamate Receptor 4

Target: Metabotropic Glutamate Receptor 4 (GRM4) (Glutamate Receptor, Metabotropic 4 (GRM4))

Alternative Name: GRM4 (GRM4 Products)

Background: Metabotropic glutamate receptor 4, GRM4, mGlu4, GPRC1D,Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1.The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2.mGluR4 is predominantly expressed presynaptically in neurons and in the cerebellum. A splice variant of the protein is expressed in taste buds. This variant lacks a large portion of the N-terminus and is normally referred to taste mGluR43,4. It is responsible (along with taste mGluR1) for mediating the taste of monosodium glutamate (or unami)2,4.mGluR4 knock out mice display impaired cerebellar synaptic plasticity as well as some learning disabilities5. In addition, this receptor has emerged as a target for the treatment of Parkinson's disease6.

Alternative names: mGluR4, Metabotropic glutamate receptor 4, GRM4, mGlu4, GPRC1D

Gene ID: 24417

NCBI Accession: NM_000841

UniProt: P31423