GNRHR antibody (Extracellular)

Catalog No. ABIN7043262

Product Details anti-GNRHR Antibody

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Binding Specificity: AA 3-13, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNRHR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to GnRH Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-GnRH Receptor (extracellular) Antibody (ABIN7043262 and ABIN7044416)) is a highly specific antibody directed against an epitope of rat GnRHR. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize GnRHR from mouse and rat samples. It is not suited to recognize GnRHR from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NNASLEQDQNH, corresponding to amino acid residues 3-13 of rat GnRHR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-GR030

Blocking Peptide: BLP-GR030

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GNRHR

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Alternative Name: GnRHR (GNRHR Products)

Background: Gonadotropin-releasing hormone receptor, GnRHR, Leutinizing hormone releasing hormone receptor, LHRHR, Luliberin receptor,Gonadotropin-releasing hormone (GnRH) or Luteinizing hormone releasing hormone (LHRH) is a decapeptide synthesized and released from neurons in the hypothalamus and stimulates the synthesis and release of gonadotropin hormone (or luteinizing hormone) and follicle stimulating hormone from the pituitary gland1. GnRH is expressed as various variants, and most vertebrates express more than one form of GnRH1-3.GnRH and its variants bind and activate GnRH receptor (GnRHR), a member of the G-protein coupled receptor (GPCR) superfamily. Three forms of the receptor have been identified in vertebrates (types I, II and III). Like all members, GnRHR has seven transmembrane domains and an extracellular N-terminus and an intracellular C-terminal tail. The receptor also has a number of glycosylation sites in its extracellular loops. In addition, the C-terminal tail is unusually short and lacks consensus sites known to undergo post-translational modifications like phosphorylation. In consequence, this affects the receptor's desensitization and internalization4.Expression of GnRHR is detected in the gonadotrope and various tissues like the breast, gonads, prostate and uterus4,5.

Alternative names: GnRH Receptor, Gonadotropin-releasing hormone receptor, GnRHR, Leutinizing hormone releasing hormone receptor, LHRHR, Luliberin receptor

Gene ID: 81668

NCBI Accession: NM_001083111

UniProt: P30969