HCN3 antibody (Extracellular)

Catalog No. ABIN7043282

Product Details anti-HCN3 Antibody

Target: HCN3 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 3 (HCN3))

Binding Specificity: AA 190-201, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to HCN3 Channel

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-HCN3 (extracellular) Antibody is directed against an epitope of rat HCN3. Anti-HCN3 (extracellular) Antibody (ABIN7043282, ABIN7044986 and ABIN7044987) can be used western blot and live cell imaging applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize HCN3 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ELEPRLDAEVYK, corresponding to amino acid residues 190-201 of rat HCN3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC

Negative Control: (ABIN7235794)

Blocking Peptide: (ABIN7235794)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HCN3

Target: HCN3 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 3 (HCN3))

Alternative Name: HCN3 (HCN3 Products)

Background: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3,Hyperpolarization-activated cation currents (Ih) appear in the heart and brain and play a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which comprises of four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2The HCN subunits can form functional homomers and can also co-assemble into functionally heteromers.2The channels are closely related to each other and share homology of about 60 % . However, they differ from each other in their cytoplasmic N- and the C-terminus. HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3The HCN3 channels are abundant in the brain where high expression of HCN3 mRNA was found in the hypothalamus.

Alternative names: HCN3, K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3

Gene ID: 114245

NCBI Accession: NM_020897

UniProt: Q9JKA8