LGI1 antibody (AA 206-218)

Catalog No. ABIN7043306

The Rabbit Polyclonal anti-LGI1 antibody has been validated for WB. It is suitable to detect LGI1 in samples from Rat.

Quick Overview for LGI1 antibody (AA 206-218) (ABIN7043306)

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LGI1 Antibody

Binding Specificity: AA 206-218

Purpose: A Rabbit Polyclonal Antibody to LGI1

Specificity: LRRCT domain

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,13 amino acid residues identical

Characteristics: Anti-LGI1 Antibody (ABIN7043306, ABIN7044578 and ABIN7044579) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize LGI1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)YKKRKINSLSPKD, corresponding to amino acid residues 206-218 of rat LGI1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235886)

Blocking Peptide: (ABIN7235886)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LGI1

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Alternative Name: LGI1

Background: Leucine-rich glioma-inactivated protein 1, Epitempin-1, EPT,The leucine-rich glioma inactivated (LGI) gene subfamily contains four highly conserved members (LGI1, 2, 3 and 4), which have been described in human, mouse and other mammalians. LGI1 is mainly expressed in the dentate gyrus and the CA3 field of the hippocampus1.The predicted structure of the LGI1 protein comprises, starting from the N-terminal, a signal peptide, four leucine-rich repeats (LRR) flanked on both sides by conserved cysteine clusters, and seven copies of a repeat of about 45 residues, named EPTP or EAR, probably forming a β-propeller structural domain. Both LRR and β-propeller domains mediate protein-protein interactions, each motif defining a distinct family of proteins.LGI1 is associated with a post-synaptic complex containing PSD-95 and ADAM22, a receptor associated with the post-synaptic membrane. Through specific binding to ADAM22, LGI1 participates in the control of synaptic strength at excitatory synapses, whose malfunction may result in epilepsy.LGI1 has been linked to several different clinical phenotypes including: malignant progression of glioma and autosomal dominant lateral temporal epilepsy, a rare familial partial epilepsy syndrome. The LGI1 gene has been shown to be frequently downregulated in malignant gliomas and to regulate invasiveness of some glioma cell lines by driving the expression of matrix metalloproteinases through the ERK 1/2 pathway. These findings suggest that LGI1 may serve as a tumor metastasis suppressor gene2. In addition, antibodies to LGI1 complexed with the KV1.1 potassium channel have been found in brain extracts from patients with limbic encephalitis3.

Alternative names: LGI1, Leucine-rich glioma-inactivated protein 1, Epitempin-1, EPT

Gene ID: 252892

NCBI Accession: NM_005097

UniProt: Q8K4Y5