LYNX1 antibody (Extracellular)

Catalog No. ABIN7043310

Product Details anti-LYNX1 Antibody

Target: LYNX1 (Ly6/Neurotoxin 1 (LYNX1))

Binding Specificity: AA 26-38, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYNX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Lynx1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: rat - 12,Mouse,13 amino acid residues identical, human - 11

Characteristics: Anti-Lynx1 (extracellular) Antibody (ABIN7043310, ABIN7044635 and ABIN7044636)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Lynx1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CAYNGDN(S)FKPMR, corresponding to amino acid residues 26-38 of mouse Lynx1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NC021

Blocking Peptide: BLP-NC021

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LYNX1

Target: LYNX1 (Ly6/Neurotoxin 1 (LYNX1))

Alternative Name: LYNX1 (LYNX1 Products)

Background: Ly-6/Neurotoxin-like protein 1, Endogenous prototoxin LYNX1,Lynx1 is a member of the Ly-6/neurotoxin gene superfamily (Lynx family) of proteins that feature a three-looped toxin fold and a cysteine rich motif, structural characteristics that are shared with the snake venom toxin α-bungarotoxin1.Lynx1 is expressed in large projection neurons in the hippocampus, cortex, and cerebellum. Initial analysis of the functional consequence of genetic ablation of Lynx1 in mice revealed that Lynx1 is an allosteric modulator of various nicotinic receptors that affects their kinetics, decreases their agonist affinities, and increases their desensitization to overall limit their activation. Lynx1 can serve as a protective measure against neurotoxicity through agonist-induced overstimulation2. In fact, deletion of Lynx1 eventually leads to neurodegeneration in aged mice3.Collectively the findings regarding Lynx1 suggest a direct relationship between plasticity regulation and nicotinic signaling, and underline the significance of fine tuning of nicotinic tone to vastly control the development of brain functions.Lynx1 activity was shown to be related to the pathophysiology of Alzheimer's disease as well as cholinergic activity in healthy and tumor cells of bronchial epithelium4.

Alternative names: Ly-6/Neurotoxin-like protein 1, Endogenous prototoxin LYNX1

Gene ID: 23936

NCBI Accession: NM_177476

UniProt: P0DP60