LPAR3 antibody (Extracellular)

Catalog No. ABIN7043314

This Rabbit Polyclonal antibody specifically detects LPAR3 in WB. It exhibits reactivity toward Rat.

Quick Overview for LPAR3 antibody (Extracellular) (ABIN7043314)

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LPAR3 Antibody

Binding Specificity: AA 16-29, Extracellular

Purpose: A Rabbit Polyclonal Antibody to LPA Receptor 3 (EDG7)

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,14 amino acid residues identical

Characteristics: Anti-LPAR3 (EDG7) (extracellular) Antibody (ABIN7043314, ABIN7044570 and ABIN7044571) is a highly specific antibody directed against an epitope to the rat LPA3 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA3 receptor from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSNTDTADEWTGTK, corresponding to amino acid residues 16-29 of rat LPA3 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235904)

Blocking Peptide: (ABIN7235904)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LPAR3

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Alternative Name: LPAR3

Background: Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32,Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptors have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2. All six receptors are type I, rhodopsin-like G-protein coupled receptors (GPCRs) that differ in their tissue distribution and downstream signaling pathways3.GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.Expression of LPA3 receptor has been observed in human heart, testis, prostate, pancreas, lung, ovary, and brain5 and is most abundant in mouse testis, kidney, lung, small intestine, heart, stomach, spleen, brain, and thymus6.LPA3 receptor null mice are viable and grossly normal, but female nulls show a striking phenotype in the reproductive system7. However, despite the fact that LPA3 is expressed in the frontal cortex, hippocampus, and amygdala, no phenotypes related to LPA3 loss in the nervous system have been reported to date5.

Alternative names: LPAR3 (EDG7), Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32

Gene ID: 66025

NCBI Accession: NM_012152

UniProt: Q8K5E0

Pathways: Regulation of Cell Size