LPAR6 antibody (Extracellular)

Catalog No. ABIN7043317

This Rabbit Polyclonal antibody specifically detects LPAR6 in WB, FACS and LCI. It exhibits reactivity toward Human.

Quick Overview for LPAR6 antibody (Extracellular) (ABIN7043317)

Target: LPAR6 (Lysophosphatidic Acid Receptor 6 (LPAR6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR6 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-LPAR6 Antibody

Binding Specificity: AA 156-168, Extracellular

Purpose: A Rabbit Polyclonal Antibody to LPA Receptor 6 (P2RY5)

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse -12,13 amino acid residues identical

Characteristics: Anti-LPAR6 (P2RY5) (extracellular) Antibody is directed against an epitope of the human LPA receptor 6. Anti-LPAR6 (P2RY5) (extracellular) Antibody (ABIN7043317, ABIN7044576 and ABIN7044577) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA receptor 6 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: STHSQGNNASEAC, corresponding to amino acid residues 156-168 of human LPA receptor 6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235906)

Blocking Peptide: (ABIN7235906)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LPAR6

Target: LPAR6 (Lysophosphatidic Acid Receptor 6 (LPAR6))

Alternative Name: LPAR6

Background: Lysophosphatidic acid receptor 6, LPA receptor 6, LPA-6, P2Y purinoceptor 5, Purinergic receptor 5, P2Y5, RB intron encoded G-protein coupled receptor,Lysophosphatidic acid (LPA, 1- or 2-acyl-sn-glycerol-3-phosphate) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptor have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2.LPA6 (P2Y5) was cloned as an orphan GPCR and is closely related to the fourth lysophosphatidic acid receptor, LPA43. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N terminus is on the extracellular side of the membrane, and the C terminus is on the cytoplasmic side4.LPA6 mRNA is expressed ubiquitously including in skin and hair follicle cells.A recent study suggested that LPA6 is essential for human hair growth. A loss of function is not compensated for, and ultimately leads to pathological changes and hair loss5.

Alternative names: LPAR6 (P2RY5), Lysophosphatidic acid receptor 6, LPA receptor 6, LPA-6, P2Y purinoceptor 5, Purinergic receptor 5, P2Y5, RB intron encoded G-protein coupled receptor

Gene ID: 10161

NCBI Accession: NM_005767

UniProt: P43657