MC1 Receptor antibody (AA 217-232)

Catalog No. ABIN7043321

Product Details anti-MC1 Receptor Antibody

Target: MC1 Receptor (MC1R) (Melanocortin 1 Receptor (MC1R))

Binding Specificity: AA 217-232

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MC1 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Melanocortin Receptor 1

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Rat

Cross-Reactivity (Details): Probably won't recognize mouse.

Predicted Reactivity: Rat - 11,6 amino acids residues identical

Characteristics: Anti-MC1 Receptor Antibody is directed against an epitope of the human melanocortin receptor 1. Anti-MC1 Receptor Antibody (ABIN7043321, ABIN7044592 and ABIN7044593)) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize MC1R from human and rat samples. The antibody is unlikely to recognize MC1R from mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HAQGIARLHKRQRPVH, corresponding to amino acid residues 217-232 of human MC1R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400-1:500

Comment:

Cited Application: IHC|ICC

Negative Control: BLP-MR020

Blocking Peptide: BLP-MR020

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MC1 Receptor

Target: MC1 Receptor (MC1R) (Melanocortin 1 Receptor (MC1R))

Alternative Name: MC1R (MC1R Products)

Background: Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2,Melanocortin receptor 1 (MC1R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The receptors ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, β- and γ-melanocyte stimulating hormone (α, β, γ-MSH) and the adrenocorticotropic hormone (ACTH) all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, that is proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.MC1R was the first member of the melanocortin receptor family to be cloned. The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP. MC1R binds its ligands with the following potency: α-MSH = ACTH > β-MSH > γ-MSH. MC1R also binds the endogenous antagonist agouti with high affinity.1, 2MC1R can be described as the "classical" melanocyte α-MSH receptor. The receptor is expressed in the skin where it has a key role in determining skin and hair pigmentation. In fur-bearing mammals, the local ratio between α-MSH and agouti will determine coat pigmentation as α-MSH stimulates and agouti inhibits melanin production.In humans, especially in Caucasians, the MC1R gene is highly polymorphic and several allelic variants have been correlated with red-hair, poor tanning ability and increased risk of melanoma.1,3,5 In addition, MC1R expression in melanoma has been shown to be upregulated up to 20-fold when compared to normal melanocytes.The melanocortins and particularly α-MSH have significant anti-inflammatory properties. Since α-MSH binds to MC1R with the highest potency, it was proposed that the latter mediated the anti-inflammatory effects of α-MSH. Indeed, MC1R expression has been demonstrated in several cells of the immune system including macrophages and neutrophils.6

Alternative names: MC1 Receptor, Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2

Gene ID: 4157

NCBI Accession: NM_002386

UniProt: Q01726

Pathways: cAMP Metabolic Process, Feeding Behaviour