Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

MC1 Receptor antibody (Cytoplasmic Domain)

MC1R Reactivity: Human, Monkey IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1049044
  • Target See all MC1 Receptor (MC1R) Antibodies
    MC1 Receptor (MC1R) (Melanocortin 1 Receptor (MC1R))
    Binding Specificity
    • 16
    • 15
    • 11
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivity
    • 59
    • 23
    • 11
    • 4
    • 2
    • 1
    • 1
    Human, Monkey
    Host
    • 61
    • 10
    Rabbit
    Clonality
    • 68
    • 3
    Polyclonal
    Conjugate
    • 30
    • 8
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This MC1 Receptor antibody is un-conjugated
    Application
    • 53
    • 29
    • 26
    • 26
    • 14
    • 11
    • 8
    • 8
    • 6
    • 4
    • 2
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human MC1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%) Baboon, Cat, Panda (94%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human MC1 Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Baboon, Cat, Panda (94%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product MC1R Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (2.5 - 5 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    MC1 Receptor (MC1R) (Melanocortin 1 Receptor (MC1R))
    Alternative Name
    MC1R (MC1R Products)
    Synonyms
    CMM5 antibody, MSH-R antibody, SHEP2 antibody, Mshra antibody, Tob antibody, e antibody, MC1-R antibody, MSHR antibody, mc1r antibody, MC1R antibody, TUBB3 antibody, melanocortin 1 receptor antibody, melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) antibody, MC1R antibody, Mc1r antibody, mc1r antibody, mc1r_par1 antibody
    Background
    Name/Gene ID: MC1R
    Subfamily: Melanocortin
    Family: GPCR

    Synonyms: MC1R, CMM5, MC1 receptor, MC1-R, MSHR, Melanocortin receptor 1, Melanotropin receptor, Melanocortin 1 receptor, MSH-R, SHEP2
    Gene ID
    4157
    Pathways
    cAMP Metabolic Process, Feeding Behaviour
You are here:
Support