MC4R antibody (Extracellular)

Catalog No. ABIN7043324

Product Details anti-MC4R Antibody

Target: MC4R (Melanocortin 4 Receptor (MC4R))

Binding Specificity: AA 6-20, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MC4R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Melanocortin Receptor 4

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - 13,15 amino acid residues identical

Characteristics: Anti-MC4 Receptor (extracellular) Antibody (ABIN7043324, ABIN7044596 and ABIN7044597) is a highly specific antibody directed against an epitope of the human melanocortin receptor 4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It recognizes an extracellular epitope and can potentially recognize the receptor in living cells. It has been designed to recognize MC4R from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HRGMHTSLHLWNRSS, corresponding to amino acid residues 6-20 of human MC4R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235928)

Blocking Peptide: (ABIN7235928)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MC4R

Target: MC4R (Melanocortin 4 Receptor (MC4R))

Alternative Name: MC4R (MC4R Products)

Background: Melanocortin receptor 4, MC4R, MC4-R,Melanocortin receptor 4 (MC4R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).1All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC4R activation is α-MSH = ACTH > β-MSH >> γ-MSH. AGRP and agouti/ASP are both endogenous antagonists of MC4R.1,2,3MC4R is widely expressed in the brain including the cortex, thalamus, hypothalamus, and brain stem. The best understood physiological role of MC4R is in the regulation of feeding behavior and the control of body weight. Indeed, mice with a targeted disruption of the MC4R gene display an obese phenotype characterized by increased adiposity, hyperphagia, and hyperleptinaemia.4 Similarly, MC4R mutations in humans have been associated with severe childhood obesity including characteristics very similar to the MC4R knockout mouse phenotype.5 All these make MC4R an attractive drug target for the treatment of obesity.

Alternative names: MC4 Receptor, Melanocortin receptor 4, MC4R, MC4-R

Gene ID: 4160

NCBI Accession: NM_005912

UniProt: P32245

Pathways: Hormone Transport, cAMP Metabolic Process, Feeding Behaviour