MCOLN1 antibody (Intracellular)

Catalog No. ABIN7043336

The Rabbit Polyclonal anti-MCOLN1 antibody has been validated for WB, IHC. It is suitable to detect MCOLN1 in samples from Mouse.

Quick Overview for MCOLN1 antibody (Intracellular) (ABIN7043336)

Target: MCOLN1 (Mucolipin 1 (MCOLN1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCOLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: KO Validated

Product Details anti-MCOLN1 Antibody

Binding Specificity: AA 6-19, Intracellular

Purpose: A Rabbit Polyclonal Antibody to TRPML1 (Mucolipin 1) Channel

Specificity: Intracellular, N-terminus (cytoplasmic)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - 12,14 amino acid residues identical

Characteristics: Anti-TRPML1 (Mucolipin 1) Antibody is directed against an epitope of mouse TRPML1. Anti-TRPML1 (Mucolipin 1) Antibody (ABIN7043336, ABIN7044039 and ABIN7044040) can be used in western blot analysis. It has been designed to recognize TRPML1 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GRRASETERLLTPN, corresponding to amino acid residues 6-19 of mouse TRPLM1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|ICC

Negative Control: (ABIN7235971)

Blocking Peptide: (ABIN7235971)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MCOLN1

Target: MCOLN1 (Mucolipin 1 (MCOLN1))

Alternative Name: MCOLN1

Background: Mcoln1, Mucolipidin,The endolysosome system takes part in important cellular functions such as membrane trafficking, protein transport, autophagy and signal transduction1. Endosomes result from endocytosis of the plasma membrane and lysosomes (which are derived from late endosomes) conatin mainly hydrolytic enzymes and generally have a low internal pH 1. Like the endoplasmic reticulum (ER), endolysosomes also store Ca2+ (luminal Ca2+ concentration: 0.5 mM)1,2, and similarly to Ca2+ release from the ER, Ca2+ from endolysosomes may also play an important role in various signaling events. To date such candidates include members of the TRP super-family of ion channels and the two-pore Ca2+ channels (TPCs)1,3,4.TRPMLs, also termed mucolipins, are members of the TRP channels. In mammals, three TRPMLs are known to date (TRPML1-3 or MCOLN1-3). They are all localized to endolysosomes, although when over expressed in heterologous systems, TRPML3 is found on the plasma membrane1,5. These channels are Ca2+ permeable and display inward rectifying current properties1,5. Like all members of this family, TRPMLs have six transmembrane domains and intracellular N- and C-termini (relatively short tails compared to other members). They are characterized by an exceptionally large extracellular (luminal) loop between transmembrane domains 1 and 2, and N-glycosylation sites are present in the first extracellular (luminal) loop5.In mammals, TRPML1 is expressed in a ubiquitous manner and shows highest expression in the brain, kidney, spleen, liver and heart1,6. TRPML2 and TRPML3 are less widely expressed. Interestingly, in mouse, two splice variants exist for TRPML2. The shorter variant is more broadly expressed and is dominant over the longer variant in the thymus, spleen and kidney1,7. TRPML3 is highly detected in the thymus, lung, kidney, spleen and eye1,7,8, some epithelial cells1,9 and brain10.Pathologies related to these channels include type IV mucolipidosis, a neurodegenetative disease characterized by retardation and retinal degeneration caused by a loss of function mutation in the gene encoding TRPML1. In contrast, a gain of function mutation in TRPML3, in mice, causes deafness, and pigmentation defects11.

Alternative names: TRPML1 (Mucolipin 1), Mcoln1, Mucolipidin

Gene ID: 94178

NCBI Accession: NM_020533

UniProt: Q99J21

Pathways: Transition Metal Ion Homeostasis