Neuregulin 3 antibody (Extracellular)

Catalog No. ABIN7043356

Product Details anti-Neuregulin 3 Antibody

Target: Neuregulin 3 (NRG3)

Binding Specificity: AA 167-180, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuregulin 3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Neuregulin-3

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - identical

Characteristics: Anti-NRG3 (extracellular) Antibody (ABIN7043356, ABIN7044722 and ABIN7044723)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Neuregulin-3 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RFPGHRVPIRASPR, corresponding to amino acid residues 167-180 of mouse Neuregulin-3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NR115

Blocking Peptide: BLP-NR115

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neuregulin 3

Target: Neuregulin 3 (NRG3)

Alternative Name: NRG3 (NRG3 Products)

Background: Neuregulin-3, HRG3,Neuregulins (NRGs) are single-pass membrane proteins belonging to a multi member family of epidermal growth factor (EGF)-like signaling molecules involved in cell-cell communication. This family includes four human neuregulin paralogs NRG1-NRG4 with multiple isoforms through alternative splicing1.Neuregulin 3 (NRG3) plays a key role in the embryonic cerebral cortex development by regulating the migration and patterning of neural progenitor cells. It specifically binds to the ErbB4 receptor tyrosine kinase in the developing mammalian forebrain2,3.Neuregulin 3 is almost exclusively expressed in the nervous system with highest expression in the anterior cingulate and frontal cortex3,4.Mutations in the neuregulin 3 gene are linked to different mental disorders accompanied with cognitive and behavioral abnormalities such as developmental delay, cognitive impairment, and psychiatric illness2,3. Single nucleotide polymorphisms are associated with delusions in patients with schizophrenia and linked to the disease itself. Knockout mice display novelty-induced hyperactivity and are deficient fear conditioning4.

Alternative names: NRG3, Neuregulin-3, HRG3

Gene ID: 18183

NCBI Accession: NM_001010848

UniProt: O35181

Pathways: RTK Signaling