Neurexin 1 antibody (Extracellular)

Catalog No. ABIN7043357

Product Details anti-Neurexin 1 Antibody

Target: Neurexin 1 (NRXN1)

Binding Specificity: AA 546-560, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurexin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Neurexin 1α

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Neurexin 1α (extracellular) Antibody (ABIN7043357, ABIN7044676 and ABIN7044677)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell imaging applications. The antibody recognizes an extracellular epitope and is therefore, highly suited to recognize neurexin 1α in living cells. It has been designed to recognize Nrxn1α from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KPRHQKDAKHPQMIK, corresponding to amino acid residues 546-560 of rat Nrxn1alpha

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: IFC

Negative Control: BLP-NR031

Blocking Peptide: BLP-NR031

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neurexin 1

Target: Neurexin 1 (NRXN1)

Alternative Name: NRXN1 (NRXN1 Products)

Background: Nrxn1, Neurexin I-alpha,Neurexins (NRXNs) are a family of transmembrane, synaptic adhesion molecules. NRXNS were identified as receptors for α-latrotoxin, a presynaptic toxin that triggers massive neurotransmitter release1. Neurexins are largely presynaptic proteins that form a trans-synaptic cell-adhesion complex with postsynaptic neuroligins2. They are encoded by three genes (NRXN1, NRXN2 and NRXN3), each using an upstream promoter to produce the longer α-isoform (α-NRXNs) and a downstream promoter to generate a shorter β-isoform (β- NRXNs).The α- and β-isoforms of each neurexin are single-pass transmembrane proteins maintaining identical transmembrane and intracellular domains but having distinct extracellular domains. NRXNs in neurons localize to the presynaptic membrane and bind trans-synaptically to postsynaptic adhesion molecules and receptors3. Neurexins are expressed in all neurons, and are subject to extensive alternative splicing, generating >1,000 splice variants, some of which exhibit highly regulated developmental and spatial expression patterns4. The extracellular domain of neurexin 1α (NRXN1α) is composed of three neurexin repeats (I, II, and III), which each contains the modules LNS-EGF-LNS. NRXN1α binds endogenous ligands including neuroligins, LRRTM family members, neurexophilin, α-dystroglycan, and GABA(A) receptors.NRXN1α gene mutations have been identified in neuropsychiatric diseases including Schizophrenia and Autism Spectrum Disorder5.

Alternative names: Neurexin 1alpha, Neurexin I-alpha, Nrxn1

Gene ID: 60391

NCBI Accession: NM_001135659

UniProt: Q63372

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development