Neuroligin 2 antibody (Extracellular)

Catalog No. ABIN7043362

Product Details anti-Neuroligin 2 Antibody

Target: Neuroligin 2 (NLGN2)

Binding Specificity: AA 657-670, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuroligin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Neuroligin 2

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,14 amino acid residues identical

Characteristics: Anti-Neuroligin 2 (extracellular) Antibody is directed against an extracellular epitope of rat Neuroligin 2. Anti-Neuroligin 2 (extracellular) Antibody (ABIN7043362, ABIN7044682 and ABIN7044683)) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NLGN2 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DLGPRAYDRFPGDS, corresponding to amino acid residues 657-670 of rat NLGN2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: IP

Negative Control: BLP-NR036

Blocking Peptide: BLP-NR036

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neuroligin 2

Target: Neuroligin 2 (NLGN2)

Alternative Name: NLGN2 (NLGN2 Products)

Background: NLGN2, NL2,Neuroligins (NLGNs) are a family of postsynaptic cell adhesion molecules that play an important role in synaptic development and function1. Four genes encode for the different members of the neuroligin family in mammals: NLGN 1-4, which are differentially enriched in postsynaptic specializations of synapses2. Neuroligin 2 (NLGN2) is expressed in neurons in the brain and also in pancreatic β cells where it facilitates insulin secretion3.Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners3. NLGN2 plays a role in synapse function and synaptic signal transmission, especially via γ-aminobutyric acid receptors (GABA(A) receptors). NLGN2 modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing4.Recent studied identified a loss-of-function mutation of NLGN2 in schizophrenia patients, suggesting an indispensable role of NLGN2 in regulating GABAergic functions5.

Alternative names: Neuroligin 2, NLGN2, NL2

Gene ID: 117096

NCBI Accession: NM_020795

UniProt: Q62888

Pathways: Cell-Cell Junction Organization, Synaptic Membrane