NPY2R antibody (Intracellular)

Catalog No. ABIN7043373

Product Details anti-NPY2R Antibody

Target: NPY2R (Neuropeptide Y Receptor Y2 (NPY2R))

Binding Specificity: AA 346-364, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPY2R antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Neuropeptide Y2 Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - identical, human - 18,19 amino acid residues identical

Characteristics: Anti-NPY2R Antibody is directed against an epitope of the mouse neuropeptide Y2 receptor (NPY2R). Anti-NPY2R Antibody (ABIN7043373, ABIN7044670 and ABIN7044671) can be used in western blot analysis, immunohistochemistry, and immunocytochemical applications. It has been designed to recognize NPY2R from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CEQRLDAIHSEVSMTFKAK, corresponding to amino acid residues 346-364 of mouse NPY2R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236094)

Blocking Peptide: (ABIN7236094)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NPY2R

Target: NPY2R (Neuropeptide Y Receptor Y2 (NPY2R))

Alternative Name: NPY2R (NPY2R Products)

Background: Neuropeptide Y2 receptor, Neuropeptide Y receptor type 2, NPY Y2 receptor,Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, together with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these, Y1, Y2, Y4, and Y5, are expressed in humans.2The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor management.2NPY2R is primarily expressed in neurons as a presynaptic receptor. It is also strongly expressed in epithelia of the visceral tissues, including colon and kidney.4 NPY2R is involved in inhibition of neurotransmitter release mediated by NPY.5 Release of glutamate, the principal neuroexcitatory transmitter, is inhibited via the NPY2R receptor.6 Involvement of NPY2R in feeding behavior was also reported. PYY(3-36), an NPY2R agonist, acts to inhibit feeding via NPY2R.7

Alternative names: NPY2R, Neuropeptide Y2 receptor, Neuropeptide Y receptor type 2, NPY Y2 receptor

Gene ID: 18167

NCBI Accession: NM_000910

UniProt: P97295

Pathways: cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour