NPY4R antibody (Intracellular)

Catalog No. ABIN7043374

Product Details anti-NPY4R Antibody

Target: NPY4R (Neuropeptide Y Receptor Y4 (NPY4R))

Binding Specificity: AA 326-340, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPY4R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Neuropeptide Y4 Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,15 amino acid residues identical

Characteristics: Anti-NPY4R Antibody is directed against an epitope of the rat neuropeptide Y4 receptor (NPY4R). Anti-NPY4R Antibody (ABIN7043374 and ABIN7044672)) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NPY4R from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: NINFKKDIKALVLTC corresponding to amino acid residues 326-340 of rat NPY4R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-NR024

Blocking Peptide: BLP-NR024

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NPY4R

Target: NPY4R (Neuropeptide Y Receptor Y4 (NPY4R))

Alternative Name: NPY4R (NPY4R Products)

Background: Neuropeptide Y4 receptor, Neuropeptide Y receptor type 4, Pancreatic polypeptide receptor 1, PPYR1,Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, along with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in the regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2NPY4R preferentially binds PP, while NPY1R, NPY2R, and NPY5R bind both NPY and PP.NPY1R, NPY2R, and NPY5R share a low degree of identity (27-31 % overall). NPY4R is more similar to NPY1R, displaying an overall identity of 43-53 %.4Human NPY4R (also named pancreatic polypeptide receptor 1) mRNA is highly expressed in the small and large intestines and the prostate. Tissue distribution studies in human and mouse suggest potential roles for NPY4R in the gastrointestinal tract, heart, and prostate, as well as in neural and endocrine signaling. Only low levels of NPY4R expression in various areas of brain were displayed.5

Alternative names: NPY4R, Neuropeptide Y4 receptor, Neuropeptide Y receptor type 4, Pancreatic polypeptide receptor 1, PPYR1

Gene ID: 29471

NCBI Accession: NM_005972

UniProt: Q63447