OXGR1 antibody (Intracellular)

Catalog No. ABIN7043418

Product Details anti-OXGR1 Antibody

Target: OXGR1 (Oxoglutarate (Alpha-Ketoglutarate) Receptor 1 (OXGR1))

Binding Specificity: AA 319-332, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OXGR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Oxoglutarate Receptor 1

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Rat - 12, human - 9

Characteristics: Anti-OXGR1/GPR99 Antibody (ABIN7043418, ABIN7044895 and ABIN7044896)) is a highly specific antibody directed against an epitope of the mouse Oxoglutarate receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize OXGR1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CKASGDLEQGKKDS, corresponding to amino acid residues 319-332 of mouse OXGR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200-1:600

Comment:

Negative Control: BLP-OR016

Blocking Peptide: BLP-OR016

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for OXGR1

Target: OXGR1 (Oxoglutarate (Alpha-Ketoglutarate) Receptor 1 (OXGR1))

Alternative Name: OXGR1 (OXGR1 Products)

Background: Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, aKGR, GPR80, P2RY15, P2Y15,Oxoglutarate receptor 1 (OXGR1) is a receptor for α-ketoglutarate (αKG), a metabolite that is a citric acid cycle intermediate and a glutamine catabolism by-product1. αKG also serves as a metabolic marker and its interaction with OXGR1 has been shown to mediate the adaptive regulation of HCO3- secretion and NaCl reabsorption under acid-base stress3.OXGR1 belongs to the G-protein coupled receptor (GPCRs) protein family. Members of this group contain seven trans-membrane domains and respond to a large variety of ligands and thereby affect key cellular functions that are crucial for cell survival or proliferation2. OXGR1 belongs to a subset of metabolic GPCRs, along with receptors for succinate (GPR91), lactate (GPR81), fatty acids (FFAR) and many more3.OXGR1 is expressed in the kidney3,4 and in the heart, where it affects the contractile function, consistent with the finding that α-ketoglutarate levels are high in heart failure patients5.Because of its major role in maintaining cellular balance and its role in heart contractile activity, identification of molecules that can specifically activate or inhibit this receptor may be very useful in the development of novel therapeutic approach for pathological cardiac hypertrophy5.

Alternative names: Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, aKGR, GPR80, P2RY15, P2Y15, OXGR1, GPR99

Gene ID: 239283

NCBI Accession: NM_080818

UniProt: Q6IYF8