Oxytocin Receptor antibody (Intracellular)

Catalog No. ABIN7043419

Product Details anti-Oxytocin Receptor Antibody

Target: Oxytocin Receptor (OXTR)

Binding Specificity: AA 346-358, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Oxytocin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Oxytocin Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-Oxytocin Receptor Antibody is directed against an epitope of the rat Oxytocin receptor. Anti-Oxytocin Receptor Antibody (ABIN7043419, ABIN7045341 and ABIN7045342)) can be used in western blot analysis. It has been designed to recognize the oxytocin receptor from rat and mouse samples. The antibody is not recommended for use with human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CSARYLKGSRPGE, corresponding to amino acid residues 346-358 of rat Oxtr

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC

Negative Control: BLP-VR013

Blocking Peptide: BLP-VR013

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Oxytocin Receptor

Target: Oxytocin Receptor (OXTR)

Alternative Name: OXTR (OXTR Products)

Background: OXTR, OT-R, OTR1,Oxytocin receptor belongs to the class 1 family of G-protein coupled receptors (GPCR) and its role is to regulate reproductive physiology, social and emotional behaviors. Like other members belonging to the GPCR superfamily, it has seven transmembrane domains, extracellular N-terminus and intracellular C-terminal tail1,2.The Oxytocin receptor ligand oxytocin, is a peptide that functions both as a hormone and as a neurotransmitter, and plays an important role in social behavior, estrous cycle, induction of labor and milk ejection, anxiety-related behaviors, food intake, stress-related responses, and pain control. Activation of the receptor leads to the stimulation of phospholipase C by interacting with Gαq/11 in response to ligand binding1.Oxytocin receptor is widely abundant in several brain regions including the basal ganglia, the limbic system, the thalamus, the hypothalamus, the brainstem, and the spinal cord. It is also detected in the uterus, mammary gland, ovary, testis, prostate and kidney.Several studies have suggested that administration of oxytocin in humans has the ability to increase generosity, trust and eye gaze. Oxytocin levels in the plasma relates to parent-child bonding behaviors, feelings of romantic love, trust and empathy. Similarly, Oxytocin receptor knockout mice shows several abnormalities in social and emotional behavior such as increased aggression and deficits in nurturing, positive affection, attachment, sensitivity and social memory2,3.

Alternative names: Oxytocin Receptor, OXTR, OT-R, OTR1

Gene ID: 25342

NCBI Accession: NM_000916

UniProt: P70536

Pathways: Myometrial Relaxation and Contraction, Feeding Behaviour