PTH2R antibody (Extracellular)

Catalog No. ABIN7043430

This anti-PTH2R antibody is a Rabbit Polyclonal antibody detecting PTH2R in WB. Suitable for Rat.

Quick Overview for PTH2R antibody (Extracellular) (ABIN7043430)

Target: PTH2R (Parathyroid Hormone 2 Receptor (PTH2R))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTH2R antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PTH2R Antibody

Binding Specificity: AA 125-137, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 11,13 amino acid residues identical

Characteristics: Anti-PTH2R (extracellular) Antibody (ABIN7043430, ABIN7045114 and ABIN7045115) is a highly specific antibody directed against an extracellular epitope of the rat Parathyroid hormone 2 receptor. The antibody can be used in western blot analysis. It has been designed to recognize PTH2 receptor from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CFLQPDINIGKQE, corresponding to amino acid residues 125-137 of rat PTH2R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:600

Comment:

Negative Control: (ABIN7236237)

Blocking Peptide: (ABIN7236237)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PTH2R

Target: PTH2R (Parathyroid Hormone 2 Receptor (PTH2R))

Alternative Name: PTH2R

Background: Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2,PTH (parathyroid hormone) regulates Ca2+ and phosphate homeostasis through its action on specific receptors in kidney and bone. There are two known PTH receptors in humans, PTH receptor type 1 (PTH1 receptor) and PTH receptor type 2 (PTH2 receptor)1.The PTH2 receptor is a member of a class B subfamily of G-protein-coupled receptors that includes the receptors for the glucagon-GHRH-VIP family of peptides (glucagon, GLP-I, GIP, GHRH, VIP, secretin, PACAP) and for calcitonin and CRF2. PTH2 receptor shares the same basic structure of the GPCR superfamily of proteins. A defining feature of the family B receptors is the relatively long extracellular N-terminal domain, which comprises about 150 amino acids and is important for ligand binding. The seven transmembrane domains are believed to be arranged in a circular or oval configuration, as seen in rhodopsin. The transmembrane domains are connected by three extracellular and three intracellular loops, and a C-terminal tail of about 130 amino acids extends intracellularly3.Northern blots of human messenger RNA (mRNA) show that the PTH2 receptor is most highly expressed in the central nervous system, preferentially in the hypothalamic regions and is also detected in the pancreas, testis, placenta, and lung4.Recent data support that PTH2 receptor is involved in hypothalamic releasing-factor secretion and pain5.

Alternative names: PTH2R, Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2

Gene ID: 81753

NCBI Accession: NM_005048

UniProt: P70555

Pathways: cAMP Metabolic Process