nectin-3 antibody (Extracellular)

Catalog No. ABIN7043441

The Rabbit Polyclonal anti-nectin-3 antibody has been validated for WB, IHC, FACS and IF. It is suitable to detect nectin-3 in samples from Mouse.

Quick Overview for nectin-3 antibody (Extracellular) (ABIN7043441)

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This nectin-3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-nectin-3 Antibody

Binding Specificity: AA 84-97, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Nectin-3/PVRL3

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - identical

Characteristics: Anti-Nectin-3/PVRL3 (extracellular) Antibody (ABIN7043441, ABIN7044696 and ABIN7044697) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Nectin-3 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ETITQISWEKIHGK, corresponding to amino acid residues 84-97 of mouse Nectin-3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235987)

Blocking Peptide: (ABIN7235987)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for nectin-3

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Alternative Name: NECTIN3

Background: Nectin cell adhesion molecule 3, Poliovirus receptor-related 3, CD113,Nectins are Ca2+-independent immunoglobulin-like cell adhesion molecules that localize at adherens junctions between two neighboring cells. They play an important role in mediating asymmetric homotypic and heterotypic cell-cell adhesions. The nectin family contains four members, nectin-1-4, which are encoded by the PVRL1-4 genes, respectively. Nectin-3 is localized mostly in dendrites and partly in axons and is crucial for the specific interactions between axons and dendrites and proper formation of the synapse1,2. Its structure consists of an extracellular region with three immunoglobulin-like loops, one V type and two C2 types.Nectins binds afadin at its c-terminal region and connects to the actin cytoskeleton. This interaction plays a major role in the formation of various cell-cell junctions in cooperation with or independently of cadherins cell adhesion molecules1,2.Nectins also regulates numerous cellular functions, such as cell movement, proliferation, polarization, survival and differentiation by interacting with various proteins1.

Alternative names: Nectin-3/PVRL3, Nectin cell adhesion molecule 3, Poliovirus receptor-related 3, CD113

Gene ID: 58998

NCBI Accession: NM_015480

UniProt: Q9JLB9

Pathways: Cell-Cell Junction Organization