PVRL4 antibody (Extracellular)

Catalog No. ABIN7043442

Product Details anti-PVRL4 Antibody

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Binding Specificity: AA 84-96, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PVRL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal antibody to Nectin-4 (extracellular).

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - 12 out of 13 amino acid residues identical Human - 11 out of 13 amino acid residues identical

Characteristics: Anti-Nectin-4 (extracellular) Antibody (ABIN7043442, ABIN7044698 and ABIN7044699)) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize Nectin-4 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KYGLHVNPAYEDR, corresponding to amino acid residues 84 - 96 of mouse Nectin-4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NR054

Blocking Peptide: BLP-NR054

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PVRL4

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: NECTIN4 (PVRL4 Products)

Background: Nectin Cell Adhesion Molecule 4, Poliovirus Receptor-Related Protein 4, PVRL4, NECTIN4,Nectin Cell Adhesion Molecule 4 (nectin-4), also known as Poliovirus Receptor-Related Protein 4 (PVRL4), is a Ca2 + -independent type 1 transmembrane protein that is a member of the immunoglobulin-like adhesion molecules protein family 1.All nectins (Nectin-1, Nectin-2, Nectin-3, and Nectin-4) share common structure features: an extracellular region of three Ig-like domains, a transmembrane region, and a cytoplasmic tail region 2.Nectin-4 interacts with afadin, a protein that has a role in cell-cell adhesion, and with nectin-1, but not with nectin-2 or 3 3.Nectin-4 was found to be overexpressed in several human cancers, including lung, ovarian, pancreatic, and breast cancer. It was also demonstrated that a soluble form of Nectin-4 is a potential diagnostic marker for several cancers and that knockdown of Nectin-4 inhibits tumor growth and reduces cell-cell contact in certain cancer types 4.Recently, an antibody-drug conjugate designed for the treatment of cancer expressing Nectin-4 was approved for use in bladder cancer patients.

Alternative names: Nectin Cell Adhesion Molecule 4, Poliovirus Receptor-Related Protein 4, PVRL4, NECTIN4

Gene ID: 71740

NCBI Accession: NM_030916

UniProt: Q8R007

Pathways: Cell-Cell Junction Organization