KCNK1 antibody (Extracellular)

Catalog No. ABIN7043443

Product Details anti-KCNK1 Antibody

Target: KCNK1 (Potassium Channel Subfamily K Member 1 (KCNK1))

Binding Specificity: AA 53-69, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to KCNK1 Channel

Specificity: Extracellular, near the P1 loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,pig - identical

Characteristics: Anti-KCNK1 (TWIK-1) (extracellular) Antibody is directed against an epitope of the human K2P1.1 channel. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (ABIN7043443, ABIN7045008 and ABIN7045009)) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting KCNK1 in living cells. It has been designed to recognize KCNK1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human KCNK1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:300

Comment:

Negative Control: BLP-PC110

Blocking Peptide: BLP-PC110

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK1

Target: KCNK1 (Potassium Channel Subfamily K Member 1 (KCNK1))

Alternative Name: KCNK1 (KCNK1 Products)

Background: Potassium channel subfamily K member 1, Inward rectifying K+ channel protein TWIK-1, K2P1.1, HOHO1, KCNO1,KCNK1 (also named TWIK-1 or K2P1.1) is a member of the 2-pore (2P) domain K+ channels family that, currently, includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, etc. but are insensitive to the classical K+ channel blockers.KCNK1 was the first of the K2P channels to be identified and as its original name indicates (Tandem of P domains in a Weak Inward rectifier K+ channel) the channel behaves as a weak inward rectifier when expressed in heterologous systems. The channel is ubiquitously expressed with the most prominent expression in the brain, kidney and heart.

Alternative names: KCNK1 (TWIK-1), Potassium channel subfamily K member 1, Inward rectifying K+ channel protein TWIK-1, K2P1.1, HOHO1, KCNO1

Gene ID: 3775

NCBI Accession: NM_002245

UniProt: O00180