KCNN4 antibody (Extracellular)

Catalog No. ABIN7044556

Product Details anti-KCNN4 Antibody

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Binding Specificity: Extracellular

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel

Specificity: Extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: KCNN4 from human,rat,and mouse samples

Characteristics: Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (ABIN7043462 and ABIN7044556) is a highly specific monoclonal antibody directed against an epitope of the human channel. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Synthetic mapping to the 3rd extracellular loop of human KCNN4

Clone: 6C1

Isotype: IgM

Application Details

Application Notes:

Antigen preadsorption control: NA

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:250

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNN4

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Alternative Name: KCNN4 (KCNN4 Products)

Background: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel,KCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel. The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.

Alternative names: KCNN4 (KCa3.1, SK4), IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Gene ID: 3783

NCBI Accession: NM_002250

UniProt: O15554