KCNN4 antibody (Internal Region)

Catalog No. ABIN6391378

This Goat Polyclonal antibody specifically detects KCNN4 in WB, ELISA, IHC, FACS and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for KCNN4 antibody (Internal Region) (ABIN6391378)

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This KCNN4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-KCNN4 Antibody

Binding Specificity: Internal Region

Purpose: KCNN4 / KCa3.1

Sequence: ERQAVNATGH LSD

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland, Kidney and Liver. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Cerebellum lysates and in lysates of cell line NIH3T3 (calculated MW of 47.7 kDa, according to Human NP_002241.1 and Mouse NP_032459.3). An additional band of unknown identity was also consistently observe

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentratio

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KCNN4

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Alternative Name: KCNN4

Background: KCNN4, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4, IK1, IKCA1, KCA4, KCa3.1, SK4, hIKCa1, hKCa4, hSK4, intermediate conductance calcium-activated potassium channel protein 1, putative erythrocyte intermediate

Gene ID: 3783, 16534, 65206

NCBI Accession: NP_002241