KCNJ6 antibody (Intracellular)

Catalog No. ABIN7043487

This anti-KCNJ6 antibody is a Guinea Pig Polyclonal antibody detecting KCNJ6 in WB, IHC and IF. Suitable for Mouse.

Quick Overview for KCNJ6 antibody (Intracellular) (ABIN7043487)

Target: KCNJ6 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 6 (KCNJ6))

Reactivity: Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This KCNJ6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-KCNJ6 Antibody

Binding Specificity: AA 374-414, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to GIRK2 (Kir3.2) Channel

Specificity: Intracellular, C-terminal domain

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - 40,41,human - 37,41 amino acid residues identical

Characteristics: Guinea pig Anti-GIRK2 (Kir3.2) Antibody  raised in guinea pigs is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GIRK2 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-GIRK2 (Kir3.2) Antibody (ABIN7043488, ABIN7044906 and ABIN7044907) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

Immunogen:

Immunogen: GST fusion protein

Immunogen Sequence: GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 of mouse Kir3.2 (GIRK2)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200-1:400

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7236407)

Blocking Peptide: (ABIN7236407)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNJ6

Target: KCNJ6 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 6 (KCNJ6))

Alternative Name: KCNJ6

Background: G protein-activated inward rectifier potassium channel 2, Kcnj6,Inward rectifying K+ (Kir) channels generate under physiological conditions, large K+ conductance at potentials negative to Ek but permit less current flow at potentials positive to Ek.Kir3.2 (also known as GIRK2 or KCNJ6) belongs to a G-protein gated subfamily of Kir channels. Kir3.2 is one of four subunits (Kir3.1, Kir3.3 and Kir3.4) that form functional tetrameric KG channel assemblies. KG channels are activated by GTP (GTPi) in the presence of an agonist or by intracellular GTPγS in the absence of an agonist. There are at least four different isoforms of Kir3.2 which are generated by alternative splicing from a single gene. Kir3.2 isoforms exhibit differential expression patterns in various tissues such as brain, pituitary, pancreas, and testis, where they usually coincide with the expression of other Kir3.x subunits.Kir3.2 is expressed on the cell surface due to two forward trafficking signal in its cytoplasmic region. One is an ER export signal in its NH2 terminus and the other is a post-ER trafficking signal that is composed of a cluster of acidic residues in the COOH terminus.Knockout of Kir3.2 is accompanied by a decrease in Kir3.1 protein expression in the brain, the development of spontaneous seizures, and a range of phenotypes related to sporadic seizures1.

Alternative names: GIRK2, G protein-activated inward rectifier potassium channel 2, Kcnj6

Gene ID: 16522

NCBI Accession: NM_002240

UniProt: P48542