KCNJ6 antibody (Intracellular)

Catalog No. ABIN7043488

This Rabbit Polyclonal antibody specifically detects KCNJ6 in WB, IHC, IF, IP and IC. It exhibits reactivity toward Mouse.

Quick Overview for KCNJ6 antibody (Intracellular) (ABIN7043488)

Target: KCNJ6 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 6 (KCNJ6))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNJ6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-KCNJ6 Antibody

Binding Specificity: AA 374-414, Intracellular

Purpose: A Rabbit Polyclonal Antibody to GIRK2 (Kir3.2) Channel

Specificity: Intracellular, C-terminal domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: golden hamster - 39,41 amino acid residues identical, human - 37,Rat - 40

Characteristics: Anti-GIRK2 (Kir3.2) Antibody (ABIN7043488, ABIN7044906 and ABIN7044907) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Kir3.2 from rat, mouse, and human samples.

Purification: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

Immunogen:

Immunogen: GST fusion protein

Immunogen Sequence: GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 of mouse Kir3.2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC|LCI

Negative Control: (ABIN7236407)

Blocking Peptide: (ABIN7236407)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNJ6

Target: KCNJ6 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 6 (KCNJ6))

Alternative Name: KCNJ6

Background: G protein-activated inward rectifier potassium channel 2, Kcnj6,Kir3.2 (or G-protein regulated inward-rectifier K+ channel, GIRK2) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir3.2 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Gα-GTP from the Gbg dimer. The latter directly binds to Kir3 and activates the channel.2,3Kir3.2 is mainly expressed in the brain, where it co-assembles with Kir3.1 (GIRK1) or Kir3.3 (GIRK3) and mediates the inhibitory effects of many neurotransmitters including opioid, adrenergic, muscarinic, dopaminergic and GABAergic neurotransmitters.2,3Point mutations in the mouse Kir3.2 channel cause the weaver (wv) phenotype, a neurological abnormality characterized by the abnormal 'weaving' of the mice when they walk, hence the name weaver which is due to a substantial loss of cerebellar granule neurons. These mice also display mild local motor hyperactivity, presumably caused by the degeneration of dopaminergic neurons in the substantia nigra, spontaneous seizures and male sterility.1A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.2 containing channels (7 nM for Kir3.2 alone and 5.4 nM for the Kir3.1/3.2 combination).4

Alternative names: GIRK2, G protein-activated inward rectifier potassium channel 2, Kcnj6

Gene ID: 16522

NCBI Accession: NM_002240

UniProt: P48542