KCNQ3 antibody (Intracellular)

Catalog No. ABIN7043505

This anti-KCNQ3 antibody is a Rabbit Polyclonal antibody detecting KCNQ3 in WB, IHC, IF, IC and IP. Suitable for Rat.

Quick Overview for KCNQ3 antibody (Intracellular) (ABIN7043505)

Target: KCNQ3 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 3 (KCNQ3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNQ3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-KCNQ3 Antibody

Binding Specificity: AA 668-686, Intracellular

Purpose: A Rabbit Polyclonal Antibody to KCNQ3 (KV7.3) Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 18,19 amino acid residues identical

Characteristics: Anti-KCNQ3 Antibody (ABIN7043505, ABIN7044961 and ABIN7044962) is a highly specific antibody directed against an epitope of the rat KV7.3 channel. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNQ3 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: AEGEKKEDNRYSDLKTIIC, corresponding to amino acid residues 668-686 of rat KCNQ3 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: (ABIN7236449)

Blocking Peptide: (ABIN7236449)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNQ3

Target: KCNQ3 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 3 (KCNQ3))

Alternative Name: KCNQ3

Background: KV7.3, Potassium voltage-gated channel subfamily KQT member 3, BFNC2,The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels. KCNQ family members can form either homomultimeric or heteromultimeric channels with different functional consequences. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor. Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as benign familial neonatal convulsions (BFNC).

Alternative names: KCNQ3, KV7.3, Potassium voltage-gated channel subfamily KQT member 3, BFNC2

Gene ID: 29682

NCBI Accession: NM_004519

UniProt: O88944