KCNA3 antibody (Extracellular) (Biotin)

Catalog No. ABIN7043522

Product Details anti-KCNA3 Antibody (Biotin)

Target: KCNA3 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 3 (KCNA3))

Binding Specificity: AA 263-276, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNA3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to Biotin 

Specificity: Extracellular loop between domains S1 and S2.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - 12,14 amino acid residues identical

Characteristics: Anti-KV1.3 (KCNA3) (extracellular) Antibody (ABIN7043521, ABIN7044992 and ABIN7044993) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse, and rat samples. \nAnti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (ABIN7043521, ABIN7044992 and ABIN7044993)-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody has been tested in western blot, immunohistochemistry and direct flow cytometry and is specially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:1000

Comment:

Negative Control: (ABIN7236496)

Blocking Peptide: (ABIN7236496)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNA3

Target: KCNA3 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 3 (KCNA3))

Alternative Name: KCNA3 (KCNA3 Products)

Background: Potassium voltage-gated channel subfamily A member 3,KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1 The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.

Alternative names: KV1.3 (KCNA3), Potassium voltage-gated channel subfamily A member 3

Gene ID: 3738

NCBI Accession: NM_002232

UniProt: P22001