Kv3.4 antibody (Intracellular)

Catalog No. ABIN7043539

Product Details anti-Kv3.4 Antibody

Target: Kv3.4 (KCNC4) (Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 4 (KCNC4))

Binding Specificity: AA 177-195, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kv3.4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to KCNC4 (KV3.4) Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - identical

Characteristics: Anti-KCNC4 (KV3.4) Antibody (ABIN7043539, ABIN7044920 and ABIN7044921) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV3.4 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EAGDD ERELA LQRLG PHEG(C), corresponding to amino acid residues 177-195 of rat KCNC4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236543)

Blocking Peptide: (ABIN7236543)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Kv3.4

Target: Kv3.4 (KCNC4) (Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 4 (KCNC4))

Alternative Name: KCNC4 (KCNC4 Products)

Background: Potassium voltage-gated channel subfamily C member 4, Raw3, KSHIIIC,KV3.4 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.1, KV3.2 and KV3.3 they constitute the Shaw type subfamily family.1As with all Kv channels, KV3.4 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits.2Kv3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.2Kv3.4 expression is wide and the channel can be found in brain, skeletal muscle, prostate and pancreas among others.KV3.4 subunits have been implicated recently in the response mechanism to chronic hypoxia and the etiology of Alzheimer's and Parkinson's diseases. In addition, KV3.4 was found to associate with the auxiliary subunit KCNE3 (MirP2) in skeletal muscle. A mutation in KCNE3 (R83H) has been associated with an inherited form of periodic paralysis (Thyrotoxic hypokalemic periodic paralysis) that is caused by the altered physiological function of the KV3.4 channel.3Toxins from sea anemone, BDS-I (#STB-400), (47 nM) and BDS-II (#B-450), (56 nM), are potent blockers of KV3.4 channels.4

Alternative names: KCNC4 (KV3.4), Potassium voltage-gated channel subfamily C member 4, Raw3, KSHIIIC

Gene ID: 684516

NCBI Accession: NM_001039574

UniProt: Q63734