KCNH1 antibody (Intracellular)

Catalog No. ABIN7043542

This Rabbit Polyclonal antibody specifically detects KCNH1 in WB, IHC, IF, IC and IP. It exhibits reactivity toward Rat.

Quick Overview for KCNH1 antibody (Intracellular) (ABIN7043542)

Target: KCNH1 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 1 (KCNH1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Immunoprecipitation (IP)

Product Details anti-KCNH1 Antibody

Binding Specificity: AA 802-817, Intracellular

Purpose: A Rabbit Polyclonal Antibody to KV10.1 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): The antibody is specific for KV10.1 and will not cross-react with KV10.2.

Predicted Reactivity: Mouse - identical, human -14,16 amino acid residues identical

Characteristics: Anti-KCNH1 Antibody is directed against an intracellular epitope of the rat KV10.1 channel. Anti-KCNH1  Antibody (ABIN7043542, ABIN7044998 and ABIN7044999) can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV10.1 from rat, human, and mouse samples. The antibody is specific for KV10.1 and will not cross-react with KV10.2.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: GDPAKRKGWARFKDAC, corresponding to amino acid residues 802-817 of rat KCNH1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236558)

Blocking Peptide: (ABIN7236558)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNH1

Target: KCNH1 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 1 (KCNH1))

Alternative Name: KCNH1

Background: KV10.1, Ether-a-go-go potassium channel 1, hEAG1,KV10.1 is a member of the Ether-a-go-go family of voltage-activated potassium channels. To date, the family includes eight members: the closely related KV10.2 (EAG-2) protein, the KV12 (elk) (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3). As with the other KV superfamily members, KV10.1 shows the characteristic 6 transmembrane domains with intracelullar N- and C-termini.The KV10 subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. Its expression is normally confined to the brain where its physiological function has not yet been clarified.Intriguingly, the KV10.1 channel has been implicated in malignant tumor development. It has been shown that KV10.1 was inappropriately expressed in several cancer cell lines. Moreover, one study showed that KV10.1 by itself had oncogenic potential, as a cell line transfected with the channel, induced aggressive and faster tumor growth in vivo as compared to a cell line transfected with an unrelated KV channel.

Alternative names: KCNH1 (EAG-1), KV10.1, Ether-a-go-go potassium channel 1, hEAG1

Gene ID: 65198

NCBI Accession: NM_002238

UniProt: Q63472