KCNH7 antibody (Intracellular)

Catalog No. ABIN7043550

Product Details anti-KCNH7 Antibody

Target: KCNH7 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 7 (KCNH7))

Binding Specificity: AA 1108-1123, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNH7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to KV11.3 (ERG-3) Channel

Specificity: Intracellular, C-terminal domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - identical, mouse - 15,16 amino acid residues identical

Characteristics: Anti-KCNH7 (erg3) Antibody (ABIN7043550 and ABIN7045010) is directed against a highly conserved epitope of the rat KV11.3 channel. The antibody will also react with human and mouse samples. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. 

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CPEFLDLEKSKLKSKE, corresponding to amino acid residues 1108-1123 of rat KV11.3 (erg3)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:300

Comment:

Cited Application: IHC

Negative Control: (ABIN7236585)

Blocking Peptide: (ABIN7236585)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNH7

Target: KCNH7 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 7 (KCNH7))

Alternative Name: KCNH7 (KCNH7 Products)

Background: KV11.3, Potassium voltage-gated channel subfamily H member 7, Ether-a-go-go-related channel 3, Eag-related protein 3, ERG-3, hERG3,KV11.3 (erg3) is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels. The erg subfamily includes the closely related proteins KV11.1 (erg1) and KV11.2 (erg2) that possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. As with all voltage-dependent K+ channels, the functional channel is a tetramer composed of four subunits. It has been suggested that the KV11 subfamily members can form functional heteromultimers within the subfamily.The current of the KV11.1 and KV11.2 channels is that of a strong inward rectifier with slow activating kinetics. The KV11.3 channel has different biophysical properties: it is a weak inward rectifier that is activated at negative potentials and has rapid activating kinetics.KV11.3 expression is believed to be concentrated mainly in the brain with the stronger expression detected in the cerebral cortex and hippocampus.Currently, there is not much pharmacological information concerning KV11.3 modulation, but the channel has been shown to be inhibited (as are all KV11 subfamily members) by the anti-arrhythmic E-4031 as well as by the anti-psychotic drugs sertindole and pimozide.

Alternative names: KCNH7 (erg3), KV11.3, Potassium voltage-gated channel subfamily H member 7, Ether-a-go-go-related channel 3, Eag-related protein 3, ERG-3, hERG3

Gene ID: 170739

NCBI Accession: NM_033272

UniProt: O54852