P2RX7 antibody (Extracellular) (FITC)

Catalog No. ABIN7043580

This anti-P2RX7 antibody is a Rabbit Polyclonal antibody detecting P2RX7 in FACS, IF, IC, LCI. Suitable for Mouse.

Quick Overview for P2RX7 antibody (Extracellular) (FITC) (ABIN7043580)

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RX7 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: KO Validated

Product Details anti-P2RX7 Antibody (FITC)

Binding Specificity: AA 136-152, Extracellular

Purpose: A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye FITC

Specificity: Extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - identical, bovine - 14,17 amino acid residues identical

Characteristics: Anti-P2X7 Receptor (extracellular) Antibody (ABIN7043578, ABIN7045080 and ABIN7045081) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples. \nAnti-P2X7 Receptor (extracellular)-FITC Antibody (ABIN7043578, ABIN7045080 and ABIN7045081)-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry and immunocytochemistry.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Cited Application: IHC|ICC|FC

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236676)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RX7

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Alternative Name: P2RX7

Background: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor,The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7. The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflamatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4

Alternative names: P2X7 Receptor, Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Gene ID: 64805

NCBI Accession: NM_002562

UniProt: Q9H244

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis