SEMA3F antibody (Secreted)

Catalog No. ABIN7043609

Product Details anti-SEMA3F Antibody

Target: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Binding Specificity: AA 280-295, Secreted

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA3F antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Semaphorin-3F

Specificity: Secreted

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Anti-Semaphorin-3F Antibody (ABIN7043609, ABIN7045296 and ABIN7045297) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize SEMA3F from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RERSAEAPQSPAVYAR, corresponding to amino acid residues 280 - 295 of human Semaphorin-3F

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7236757)

Blocking Peptide: (ABIN7236757)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SEMA3F

Target: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Alternative Name: SEMA3F (SEMA3F Products)

Background: Semaphorin IV, SEMA3F,Semaphorins are a large family of proteins with over 30 members, which are further divided in subgroups1. They are involved in signaling and are implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration2. They are highly expressed in mammary gland, kidney, fetal brain, and lung and expressed at lower levels in heart and liver tissues2.All semaphorin members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues3. The semaphorin 3 family have a basic domain at the C-terminus. They bind neuropilins which subsequently activate signaling through associated plexins. Semaphorin-3B and semaphorin-3F function additionally as potent inhibitors of tumor development in small cell lung carcinoma. Recent evidence indicates that these semaphorins modulate the adhesive and migratory properties of responsive malignant cells. Semaphorin-3F was also functions as an inhibitor of angiogenesis, contributing significantly to its anti-tumorigenic properties1.

Alternative names: Semaphorin IV, SEMA3F

Gene ID: 6405

NCBI Accession: NM_004186

UniProt: Q13275

Pathways: Regulation of Cell Size