Sema4a antibody (Extracellular)

Catalog No. ABIN7043610

Product Details anti-Sema4a Antibody

Target: Sema4a (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4A (Sema4a))

Binding Specificity: AA 355-369, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sema4a antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal antibody to Semaphorin 4A (extracellular).

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (ABIN7043610, ABIN7045300 and ABIN7045301) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Semaphorin 4A from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KELNKETSRWTTYRG, corresponding to amino acid residues 355 - 369 of human Semaphorin 4A

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7236761)

Blocking Peptide: (ABIN7236761)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Sema4a

Target: Sema4a (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4A (Sema4a))

Alternative Name: SEMA4A (Sema4a Products)

Background: SEMAB,Neuroimmune Semaphorin 4A (Sema4A) is a member of Semaphorin family of transmembrane and secreted proteins.The Semaphorin protein family members regulate the functional activity of axons in the nervous system, and Sema4A is an important regulator of neuronal and immune functions 1,2. In accordance to that, it was shown to be preferentially expressed on dendritic cells and B cells in the immune system 3.Sema4A consists of an NH2-terminal signal peptide followed by a Sema domain, an Ig domain of the C2 type, a hydrophobic transmembrane region, and a cytoplasmic tail 2, it has seven currently known receptors through which it mediates a complex system of intracellular and extracellular signals that regulate different physiological and pathological tissue processes 1. In the nervous system, Sema4A serves as an axon guidance molecule and in the immune system it regulates immune cell activation and function, instructing the fine tuning of the immune response 1.Recent studies have shown a dysregulation of Sema4A expression in several types of cancer such as hepatocellular carcinoma, colorectal, and breast cancers 1,4. The function of Sema4A in angiogenesis and cancer is not defined but it is shown to be related to the process of angiogenesis. Understanding the role of Sema4A in pathologic processes underlining tumorigenesis and tumor metastasis may guide the development of improved therapeutic treatments for cancer 1.

Alternative names: SEMAB

Gene ID: 64218

NCBI Accession: NM_022367

UniProt: Q9H3S1