SEMA4D/CD100 antibody (Extracellular)

Catalog No. ABIN7043611

This Rabbit Polyclonal antibody specifically detects SEMA4D/CD100 in WB, FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for SEMA4D/CD100 antibody (Extracellular) (ABIN7043611)

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA4D/CD100 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-SEMA4D/CD100 Antibody

Binding Specificity: AA 80-93, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Semaphorin 4D

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-Semaphorin 4D (SEMA4D) (extracellular) Antibody (ABIN7043611, ABIN7045298 and ABIN7045299) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SEMA4D from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EKQHEVYWKVSEDK, corresponding to amino acid residues 80-93 of mouse SEMA4D

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236765)

Blocking Peptide: (ABIN7236765)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SEMA4D/CD100

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name: SEMA4D

Background: CD100, Semaphorin-J,Semaphorins are a family of soluble and membrane associated proteins characterized by a conserved "sema" domain. They were originally described as axonal growth and guidance proteins required to guide neuronal axons to the right targets. Today semaphorins are known to possess pleiotropic and important functions in several physiological and pathological processes, including heart development, vascular growth, tumor progression, immune cell regulation, angiogenesis and vasculogenesis. To date, more than 20 types of semaphorin proteins have been identified and classified into eight subclasses1,2. The conserved "sema" domain of the protein includes arginine-glycine-aspartate sequences. The C-terminal region includes an IgG-like domain, a transmembrane domain, and a short cytoplasmic tail that contains one tyrosine phosphorylation site and multiple sites for serine-threonine phosphorylation1,2.Semaphorin 4D (Sema4D), also known as CD100, is a 150- kDa membrane-associated-type class IV semaphorin and was the first semaphorin shown to have immune regulatory activity. Other Sema4D functions include inactivation of platelets, stimulation of angiogenesis, and regulation of bone formation. Sema4D is expressed in many tissues including the brain, kidney, and heart. Sema4D is expressed in resting T cells, and expression is increased upon cellular activation3.

Alternative names: Semaphorin 4D (SEMA4D), CD100, Semaphorin-J

Gene ID: 20354

NCBI Accession: NM_006378

UniProt: O09126

Pathways: Regulation of Cell Size