SCN3A antibody (Extracellular)

Catalog No. ABIN7043644

This Rabbit Polyclonal antibody specifically detects SCN3A in WB, IF, IC and LCI. It exhibits reactivity toward Rat.

Quick Overview for SCN3A antibody (Extracellular) (ABIN7043644)

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCN3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-SCN3A Antibody

Binding Specificity: AA 277-288, Extracellular

Purpose: A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel

Specificity: 3rd extracellular loop, domain I.

Cross-Reactivity (Details): May recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).

Predicted Reactivity: Mouse,human - 11,12 amino acid residues identical

Characteristics: Anti-SCN3A (Nav1.3) (extracellular) Antibody is directed against an epitope of the rat NaV1.3 channel. Anti-SCN3A (NaV1.3) (extracellular) Antibody (ABIN7043644, ABIN7045250 and ABIN7045251) can be used in western blot, and live cell imaging applications. It has been designed to recognize NaV1.3 from rat, human and mouse samples. The antibody may recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CSQWPPSDSAFE, corresponding to amino acid residues 277-288 of rat NaV1.3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC|FC

Negative Control: (ABIN7236847)

Blocking Peptide: (ABIN7236847)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SCN3A

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Alternative Name: SCN3A

Background: BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha,Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7 NaV1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of NaV1.3 channel expression was described in injured neurons and injured spinal cord.9-11

Alternative names: SCN3A (NaV1.3), BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha

Gene ID: 497770

NCBI Accession: NM_006922

UniProt: P08104