SCN5A antibody (Intracellular)

Catalog No. ABIN7043650

Product Details anti-SCN5A Antibody

Target: SCN5A (Sodium Channel, Voltage-Gated, Type V, alpha Subunit (SCN5A))

Binding Specificity: AA 493-511, Intracellular

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This SCN5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Guinea Pig Polyclonal Antibody to NaV1.5 (SCN5A) (493-511) Channel

Specificity: Intracellular loop between domains I and II

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 17,19 amino acid residues identical

Characteristics: Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NaV1.5 sodium channel from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-NaV1.5 (SCN5A) (493-511) Antibody (#) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: DRLPKSDSEDGPRALNQLSC, corresponding to amino acid residues 493-511 of rat NaV1.5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-SC005

Blocking Peptide: BLP-SC005

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SCN5A

Target: SCN5A (Sodium Channel, Voltage-Gated, Type V, alpha Subunit (SCN5A))

Alternative Name: SCN5A (SCN5A Products)

Background: Sodium channel protein type 5 subunit alpha, SKM2,Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability1.Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive NaV1.52.The putative structure of NaV1.5 consists of four homologous domain (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome1,2. Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system1.

Alternative names: NaV1.5, Sodium channel protein type 5 subunit alpha, SKM2

Gene ID: 25665

NCBI Accession: NM_198056

UniProt: P15389