SLC16A7 antibody (Intracellular)

Catalog No. ABIN7043678

The Rabbit Polyclonal anti-SLC16A7 antibody has been validated for WB, IHC and IF. It is suitable to detect SLC16A7 in samples from Rat.

Quick Overview for SLC16A7 antibody (Intracellular) (ABIN7043678)

Target: SLC16A7 (Solute Carrier Family 16, Member 7 (Monocarboxylic Acid Transporter 2) (SLC16A7))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC16A7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SLC16A7 Antibody

Binding Specificity: AA 456-468, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 2

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Mouse - 10,13 amino acid residues identical

Characteristics: Anti-MCT2 Antibody (ABIN7043678, ABIN7044616 and ABIN7044617) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MCT2 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEMEALSRSKQDD, corresponding to amino acid residues 456-468 of rat MCT2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236948)

Blocking Peptide: (ABIN7236948)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC16A7

Target: SLC16A7 (Solute Carrier Family 16, Member 7 (Monocarboxylic Acid Transporter 2) (SLC16A7))

Alternative Name: SLC16A7

Background: Monocarboxylate transporter 2, Monocarboxylic acid transporter 2, SLC16A7,The SLC16A family of monocarboxylate transporters (MCTs) is composed of 14 members. MCTs play a central role in cellular metabolism and metabolic communication between tissues. The rapid transport of monocarboxylates such as pyruvate, lactate, and ketone bodies (acetoacetate and β-hydroxybutyrate) across the plasma membrane of cells is facilitated by proton-linked MCTs1. MCTs share similarities in sequence and protein structure, but each member differs from others in the substrates that they transport. For example, lactate among other monocarboxylates, is the primary substrate for MCT1, MCT2, MCT3 and MCT42. All family members are predicted to have 12 transmembrane helices (TMs) with intracellular C- and N-termini as well as a large cytosolic loop3.MCT2 is distributed in testis, brain, skeletal muscle, heart, and kidney4.MCTs are important for normal metabolic function and are implicated in disease. Changes in the cerebral density of MCT2 have been reported acutely following hypoxia-ischemia in vivo and in hippocampal slices5.

Alternative names: MCT2, Monocarboxylate transporter 2, Monocarboxylic acid transporter 2, SLC16A7

Gene ID: 29735

NCBI Accession: NM_004731

UniProt: Q63344