SLC2A5 antibody (Intracellular)

Catalog No. ABIN7043702

The Rabbit Polyclonal anti-SLC2A5 antibody has been validated for WB, IHC and IF. It is suitable to detect SLC2A5 in samples from Rat.

Quick Overview for SLC2A5 antibody (Intracellular) (ABIN7043702)

Target: SLC2A5 (Solute Carrier Family 2 (Facilitated Glucose/fructose Transporter), Member 5 (SLC2A5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC2A5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SLC2A5 Antibody

Binding Specificity: AA 477-490, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Glucose Transporter 5

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Mouse - 12, human - 11

Characteristics: Anti-GLUT5 (SLC2A5) Antibody (ABIN7043702, ABIN7044489 and ABIN7044490) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 5 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT5 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KNKVSDVYPEKEEK, corresponding to amino acid residues 477 - 490 of rat Glucose Transporter 5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7237009)

Blocking Peptide: (ABIN7237009)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC2A5

Target: SLC2A5 (Solute Carrier Family 2 (Facilitated Glucose/fructose Transporter), Member 5 (SLC2A5))

Alternative Name: SLC2A5

Background: Glucose transporter 5, Facilitated glucose transporter member 5, Fructose transporter,The GLUT protein family belongs to the solute carrier 2 family (SLC2) and is a member of the major facilitator superfamily of membrane transporters. The GLUT proteins have a fold consisting of two symmetrical six transmembrane helix (TM) bundle. In addition, the proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini, and a several conserved residues and motifs designated "sugar transporter signatures"1,2.Fourteen GLUT isoforms have been identified and are divided into 3 different group classes based on sequence similarity and structural and functional characteristics. GLUT 5,7,9 and 11 belong to class ll group1.The GLUT transporters are uniporters, which facilitate the diffusion of monosaccharides like glucose and fructose across the cell membrane in a concentration-dependent manner. GLUT5 is specific to fructose and is primarily expressed in the small intestine, but lower levels were also detected in the brain, adipose tissue, kidney, testes and skeletal muscle. Several metabolic disorders and cancers are linked to increased GLUT5 expression2.

Alternative names: GLUT5 (SLC2A5), Glucose transporter 5, Facilitated glucose transporter member 5, Fructose transporter

Gene ID: 65197

NCBI Accession: NM_003039

UniProt: P43427