SLC22A4 antibody (Intracellular)

Catalog No. ABIN7043704

Product Details anti-SLC22A4 Antibody

Target: SLC22A4 (Solute Carrier Family 22 Member 4 (SLC22A4))

Binding Specificity: AA 522-536, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC22A4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal antibody to OCTN1/SLC22A4 transporter.

Specificity: Intracellular, C-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 14 out of 15 amino acid residues identical, Human - 12 out of 15 amino acid residues identical

Characteristics: Anti-OCTN1/SLC22A4 Antibody (ABIN7043704, ABIN7044225 and ABIN7044226) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize OCTN1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EQMQKVRGFR(S)GKKS, corresponding to amino acid residues 522 - 536 of mouse OCTN1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200

Application Dilutions Western blot wb: 1:200-1:600

Comment:

Cited Application: IHC

Negative Control: (ABIN7237017)

Blocking Peptide: (ABIN7237017)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC22A4

Target: SLC22A4 (Solute Carrier Family 22 Member 4 (SLC22A4))

Alternative Name: SLC22A4 (SLC22A4 Products)

Background: Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4,Organic Cation Transporter Novel (OCTN) is a subfamily of the larger Organic Cation Transporter (OCT) or SLC22 transporter family.The OCTN subfamily consists of two members in humans: OCTN1 (SLC22A4) and OCTN2 (SLC22A5) with a third member (SLC22A21) present only in rodents 1-2.The physiological role of OCTN1 (SLC22A4) has not yet been elucidated. OCTN1 is ubiquitously expressed throughout the body and transports various therapeutic agents, including organic cations and zwitterions. Ergothioneine (ET/ ERGO) a natural diet-derived antioxidant was found to be a specific substrate of OCTN1 3.OCTN1 is functionally expressed in neurons and neural stem cells and activation of the transporter could conceivably have a protective role in neurodegenerative diseases. In addition, in peripheral organs, mutations in OCTN1 are associated with susceptibility to Crohn's disease and rheumatoid arthritis suggesting that OCTN1 may play a role in inflammatory dieseases 1.

Alternative names: Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4

Gene ID: 30805

NCBI Accession: NM_003059

UniProt: Q9Z306