SLC39A1 antibody (Extracellular)

Catalog No. ABIN7043723

Product Details anti-SLC39A1 Antibody

Target: SLC39A1 (Solute Carrier Family 39 (Zinc Transporter), Member 1 (SLC39A1))

Binding Specificity: AA 5-20, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC39A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Zinc Transporter ZIP1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 15,16 amino acid residues identical

Characteristics: Anti-ZIP1 (SLC39A1) (extracellular) Antibody is directed against an epitope of the human Zinc transporter ZIP1. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (ABIN7043723, ABIN7045344 and ABIN7045345) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize ZIP1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GEPELLVWRPEAVASE, corresponding to amino acid residues 5-20 of human ZIP1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237087)

Blocking Peptide: (ABIN7237087)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC39A1

Target: SLC39A1 (Solute Carrier Family 39 (Zinc Transporter), Member 1 (SLC39A1))

Alternative Name: SLC39A1 (SLC39A1 Products)

Background: Zinc transporter ZIP1, Zinc-iron-regulated transporter-like, Zrt- and Irt-like protein 1, ZIRTL,Zinc uptake transporter, ZIP1, belongs to the SLC39 transporter family. This transport has the ability to increase zinc concentration when cellular zinc level is low, by active zinc transport into the cytoplasm from extracellular space of the cell or the intracellular storage compartment. Thus, zinc transport plays a crucial role in regulating cellular zinc homeostasis1-3.Regulation and maintenance of proper concentration and distribution of cellular zinc are essential to the function, metabolism, growth, proliferation and survival of cells. Zinc deficiency can cause defects in the immune system, including abnormalities in T-cells, natural killer cells and monocytes, and reduction in antibody formation1,2.In the human genome, 20 ZIP transporters have been identified. ZIP1 is encoded by the SLC39A1 gene3.ZIP proteins are highly conserved. Members have eight predicted transmembrane (TM) domains with extracellular N- and C-termini. There is a long loop region harboring a histidine-rich domain between TM3 and TM4. Zinc uptake by ZIP1 is energy independent2.ZIP1 is ubiquitously expressed in human tissues including blood, brain, gill, heart, intestine, liver, muscle, skin, spleen and kidney3.Accumulation of zinc in prostate cells by ZIP1 inhibits their growth and proliferation. Therefore, ZIP1 is associated as a tumor suppressor gene in prostate cancer2.

Alternative names: ZIP1 (SLC39A1), Zinc transporter ZIP1, Zinc-iron-regulated transporter-like, Zrt- and Irt-like protein 1, ZIRTL

Gene ID: 27173

NCBI Accession: NM_014437

UniProt: Q9NY26

Pathways: Autophagy