SLC5A8 antibody (Intracellular)

Catalog No. ABIN7043734

This Rabbit Polyclonal antibody specifically detects SLC5A8 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for SLC5A8 antibody (Intracellular) (ABIN7043734)

Target: SLC5A8 (Solute Carrier Family 5 (Iodide Transporter), Member 8 (SLC5A8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC5A8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SLC5A8 Antibody

Binding Specificity: AA 544-558, Intracellular

Purpose: A Rabbit Polyclonal Antibody to SMCT1 (SLC5A8) Transporter

Specificity: Intracellular, C-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 13 out of 15 amino acid residues identical

Characteristics: Anti-SMCT1 (SLC5A8) Antibody (ABIN7043734, ABIN7044495 and ABIN7044496) is a highly specific antibody directed against an epitope of the human SLC5A8. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC5A8 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of  mouse beta-2-Adrenoceptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237129)

Blocking Peptide: (ABIN7237129)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC5A8

Target: SLC5A8 (Solute Carrier Family 5 (Iodide Transporter), Member 8 (SLC5A8))

Alternative Name: SLC5A8

Background: Sodium-Coupled Monocarboxylate Transporter 1, Solute Carrier Family 5 Member 8, Apical Iodide Transporter, AIT,Sodium-coupled monocarboxylate transporter 1, SLC5A8, is an electrogenic sodium (Na+) and chloride (Cl-)-dependent sodium-coupled solute transporter that belongs to the Na+/glucose co-transporter gene family (SLC5) 1.It is expressed in the colon, ileum, kidney and thyroid gland and it is involved in the transport of a variety of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate) 1.SLC5A8 was found to be silenced in colon cancer, gastric cancer and possibly other cancer types and hence it is a candidate tumor suppressor gene. This is probably mediated through its role in transforming butyrate, a metabolite that is related to these types of cancer 2.The structure of SLC5A8 was predicted based on its homology to sodium-iodide symporter (NIS) and on the fact that it is a part of the Solut Sodium Symporter (SSS) protein family. The structure contains 13 α-helical TM segments, with the N-terminus facing the extracellular medium and the C-terminus facing the cytoplasm, and a conserved sodium binding site 3.

Alternative names: Sodium-Coupled Monocarboxylate Transporter 1, Solute Carrier Family 5 Member 8, Apical Iodide Transporter, AIT

Gene ID: 160728

NCBI Accession: NM_145913

UniProt: Q8N695