Slc6a14 antibody (Extracellular)

Catalog No. ABIN7043737

This Rabbit Polyclonal antibody specifically detects Slc6a14 in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Slc6a14 antibody (Extracellular) (ABIN7043737)

Target: Slc6a14 (Solute Carrier Family 6 (Amino Acid Transporter), Member 14 (Slc6a14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Slc6a14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Slc6a14 Antibody

Binding Specificity: AA 213-226, Extracellular

Purpose: A Rabbit Polyclonal Antibody to SLC6A14 Transporter

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical, rat - 11,Mouse - 12

Characteristics: Anti-SLC6A14 (extracellular) Antibody (ABIN7043737, ABIN7044477 and ABIN7044478) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC6A14 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)PSEQYWNKVALQRS, corresponding to amino acid residues 213-226 of human SLC6A14

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237141)

Blocking Peptide: (ABIN7237141)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Slc6a14

Target: Slc6a14 (Solute Carrier Family 6 (Amino Acid Transporter), Member 14 (Slc6a14))

Alternative Name: SLC6A14

Background: Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14,SLC614A is an amino acid transporter which is a member of the Solute carrier (SLC) 6 transporter family. Currently, the SLC6 family is comprised of >300 prokaryotic and eukaryotic proteins, of which 21 have been identified in humans. SLC6 members have 12 membrane spanning domains (TM) with intracellular N- and C- termini1.SLC614A transports all amino acids (except glutamate and aspartate) into the cell. It has a uni-directional function and it is energized by three different driving forces: a Na+ gradient, a Cl- gradient and the membrane potential. These specific characteristics makes it important in the development of cancerous tumors2. During carcinogenesis, the developing tumor utilizes more resources than healthy tissue and, therefore, there is an over-production of transporters for glucose and amino acids. SLC614A is uniquely suited to the biological needs of tumor cells: Theoretically, this transporter has the ability to concentrate its substrates inside the cell ~1000-fold compared to the extracellular medium, which complies with the increasing demand of the tumor cell for amino acid supply2. Indeed, SLC614A has been shown to be up regulated in multiple cancer types such as, cervical cancer3, breast cancer4, and pancreatic cancer5, as well as the development of Cystic fibrosis6. Hence, the therapeutic potential in affecting the activity of this transporter is clinically significant.

Alternative names: SLC6A14, Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14

Gene ID: 11254

NCBI Accession: NM_007231

UniProt: Q9UN76