SLC6A13 antibody (Intracellular)

Catalog No. ABIN7043743

This Rabbit Polyclonal antibody specifically detects SLC6A13 in IHC, WB and IF. It exhibits reactivity toward Rat.

Quick Overview for SLC6A13 antibody (Intracellular) (ABIN7043743)

Target: SLC6A13 (Solute Carrier Family 6 (Neurotransmitter Transporter, GABA), Member 13 (SLC6A13))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A13 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-SLC6A13 Antibody

Binding Specificity: AA 23-38, Intracellular

Purpose: A Rabbit Polyclonal Antibody to GABA Transporter 2 (GAT-2)

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 16 amino acid residues identical, human - 11,Mouse - 14

Characteristics: Anti-GABA Transporter 2 Antibody is directed against an epitope of rat GABA transporter 2. Anti-GABA Transporter 2 Antibody (ABIN7043743, ABIN7044461 and ABIN7044462) can be used in western blot analysis and immunohistochemistry applications and has been designed to recognize from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KVEEDGTLEREQWTNK, corresponding to amino acids residues 23-38 of rat GAT-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237161)

Blocking Peptide: (ABIN7237161)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC6A13

Target: SLC6A13 (Solute Carrier Family 6 (Neurotransmitter Transporter, GABA), Member 13 (SLC6A13))

Alternative Name: SLC6A13

Background: SLC6A13, GABT2, Na+/Cl-- dependent GABA transporter 2,γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3.GAT-2 is expressed in the brain and in the periphery and is also abundant in the kidney and liver4. Due to its relatively broad expression pattern, suggested roles for GAT-2 include maturation of the nervous system, and regulation of GABA levels among others4.

Alternative names: GABA Transporter 2 (GAT-2), SLC6A13, GABT2, Na+/Cl-- dependent GABA transporter 2

Gene ID: 171163

NCBI Accession: NM_016615

UniProt: P31646