SLC9A1 antibody (Extracellular)

Catalog No. ABIN7043761

Product Details anti-SLC9A1 Antibody

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Binding Specificity: AA 54-67, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 1 (NHE-1)

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human,pig,dog - 13,14 amino acid residues identical

Characteristics: Anti-Na+/H+ Exchanger 1 Antibody (ABIN7043761, ABIN7044873 and ABIN7044874) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RERSIGDVTTAPSE, corresponding to amino acid residues 54-67 of rat NHE-1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: ICC

Negative Control: (ABIN7237223)

Blocking Peptide: (ABIN7237223)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC9A1

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Alternative Name: SLC9A1 (SLC9A1 Products)

Background: Sodium/hydrogen exchanger 1, SLC9A1, APNH,In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH . They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1. To date, nine genes have been identified in mammals, NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4. Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6. K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH . Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH , they are rapidly activated1,5,8. NHE-1 expression is ubiquitous and may serve as a housekeeping gene9.

Alternative names: Na+/H+ Exchanger 1 (NHE-1), Sodium/hydrogen exchanger 1, SLC9A1, APNH

Gene ID: 24782

NCBI Accession: NM_003047

UniProt: P26431

Pathways: Glycosaminoglycan Metabolic Process, Proton Transport