SLC9A1 antibody

Catalog No. ABIN3197494

Cited in 1 publication. The Rabbit Polyclonal anti-SLC9A1 antibody (ABIN3197494) specifically detects SLC9A1 in WB and ELISA. The antibody is reactive with Vitis vinifera, Tomato, Oryza sativa, Kandelia obovata and Arabidopsis thaliana samples.

Quick Overview for SLC9A1 antibody (ABIN3197494)

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Reactivity: Vitis vinifera, Tomato, Oryza sativa, Kandelia obovata, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SLC9A1 Antibody

Purpose: Anti-Na+/H+ antiporter, sodium/hydrogen exchanger

Cross-Reactivity (Details): Arabidopsis thaliana, Kandelia obovata, Oryza sativa, Solanum lycopersicum, Vitis vinifera
Not reactive in: Mangrove plants, Nicotiana benthamiana, sp. Avicennia,

Predicted Reactivity: Gossypium hirsutum,Populus euphratica,Ricinus communis,Zea mays (NHX1,NHX4)

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NHX protein UniProt: Q68KI4

Application Details

Application Notes: 1 : 8000 (ELISA), 1 : 1000 (WB)

Comment:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20°C to -80°C.Manufacture by Operon Biotechnologies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-SLC9A1 antibody (ABIN3197494)

Chen, Xiao, Wu, Dong, He, Pei, Zheng: "Nitric oxide enhances salt secretion and Na(+) sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H(+)-ATPase and Na(+)/H(+) antiporter under high salinity." in: Tree physiology, Vol. 30, Issue 12, pp. 1570-85, (2010) (PubMed).

Target Details for SLC9A1

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Alternative Name: NHE-1

Background:

Background: Na(+)/H(+) exchanger 1 protein is involved in exchange of protons for cations such as sodium and potassium across membranes. Localized in tonoplast, possibly also to ER and Golgi. Alternative name: NHE-1, Na(+)/H(+) exchanger 1

Additional information: Protocol for vacuolar membrane isolation can be found here.

Molecular Weight: 59.5 | 45 kDa

Gene ID: 832773

UniProt: Q68KI4

Pathways: Glycosaminoglycan Metabolic Process, Proton Transport